DNA content and ploidy estimation of Festuca ovina accessions by flow cytometry
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چکیده
منابع مشابه
Estimation of Nuclear DNA Content and Determination of Ploidy Level in Tunisian Populations of Atriplex halimus L. by Flow Cytometry
Kheiria Hcini1, David J. Walker2, Elena González3, Nora Frayssinet4, Enrique Correal2 and Sadok Bouzid1 1Faculté des Sciences de Tunis, Département des Sciences Biologiques, Tunis, 2Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), C/Mayor, s/n 30150 La Alberca, Murcia, 3Facultad de Ciencias Exactas y Naturales, UNPSJB – Comodoro Rivadavia, 4Facultad de Agronomía, ...
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Background & Aims: Gastric cancer is the second common cancer in Iran and it has unfortunately no diagnostic signs in early stages. Considering the role of genetic and environmental factors in the development of gastric cancer, this study aimed at evaluating DNA-content of stomach cells in patients with gastric cancer in order to find a reliable index for prediction of disease prognosis and pro...
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Nuclear DNA flow cytometry was used to differentiate ploidy level and determine nuclear DNA content in Rubus. Nuclei suspensions were prepared from leaf discs of young leaves following published protocols with modifications. DNA was stained with propidium iodide. Measurement of fluorescence of 40 genotypes, whose published ploidy ranged from diploid to dodecaploid, indicated that fluorescence i...
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During recent years, flow cytometry has established itself as a useful, quick novel method to determine efficiently, reproducibly and at a reduced cost per sample the relative nuclear DNA content and ploidy level of a large number of species (plants and animals), that has also been used to sort cells with different traits from a mixed cell population (e.g. as when separating heterokaryons from ...
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Erianthus arundinaceus is not only an important germplasm resource for sugarcane breeding but also a potential bioenergy plant. Making clear the distribution of the chromosome ploidy of wild E. arundinaceus in china is the premise of the research and utilization of this species. Therefore, the objectives of this study were to determine the ploidy level and DNA content of the 55 E. arundinaceus ...
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ژورنال
عنوان ژورنال: Crop Science
سال: 2020
ISSN: 0011-183X,1435-0653
DOI: 10.1002/csc2.20229